Amusan’s 12.60s return time was not enough to earn her a medal.
With record 18 games unbeaten, Akwa United had the longest unbeaten run in the league and clean sheets this season.
The red hot Osimhen led Napoli’s attack brilliantly on the day, giving Napoli the lead in the 69th minute courtesy of an assist from Adam Ounas.
She will run from lane six against seven other qualifiers on Monday.
Enoch Adegoke has crashed out of the Tokyo Olympics 100m men’s final as he pulled hamstring metres away from the finish line.
Nigeria last qualified for Olympics 100m finals at Atlanta ’96 via Davidson Ezenwa.
The coach had earlier been tested and confirmed to have high blood pressure by organisers of the competition.
Djokovic is yet to get an Olympic gold, his best being a 2008 bronze.
Nigeria did not meet the minimum testing requirements under Rule 15 for 10 athletes.
Itsekiri will now appear in Sunday’s first semi-final, while Adegoke will compete in the second semi-final.
